Impact of Intrauterine Growth Restriction Diseases on The Umbilical Cord Blood CD34+ Cell Counts

Morbidly Adherent Placenta and umbilical cord blood CD34+ cells


Abstract views: 263

Authors

  • Enes Mehmet Türkoğlu
  • Şerife Erdem
  • Fatma Zehra Okus
  • Alper Özcan
  • Zehra Büşra Azizoğlu
  • Ahmet Eken
  • Semih Zeki Uludağ
  • Mehmet Serdar Kutuk
  • Musa Karakukcu
  • Ekrem Ünal

Abstract

Introduction: Different diseases in obstetrics and gynecology can affect the number of CD34+ cells in the umbilical cord blood.
Objectives: This study aimed to evaluate the effect of Gestational Diabetes Mellitus (GDM), Gestational Hypertension (GHT) and Morbidly Adherent Placenta (MAP) on the content of CD34+ cells of umbilical cord blood and to compare the effectiveness of Sysmex XN20 analyzer to the flow cytometry method, which is the gold standard in CD34+ cells.
Materials abd Methods: The umbilical cord blood (15 ml) was collected after the birth of the newborns. Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque Plus. The cells were stained with Procount ™ Progenitor Cell Count Kit with PE Labeled monoclonal anti-CD34+ antibody then analyzed with Flow Cytometry or Sysmex XN20 without staining, to identify CD34+ cells named as hematopoietic progenitor cells (HPC), respectively.
Results: Flow cytometric evaluation revealed a significantly elevated (p<0.05) number of cord blood CD34+ cells in GDM and GHT groups compared with healthy controls. MAP patients had comparable CD34+ cells compared with healthy controls. A significant increase in lymphocyte counts was also observed in GDM and GHT groups compared with healthy controls. Sysmex analysis however only revealed an increase in lymphocyte numbers in GHT but picked no differences across groups in HPC. Correlation between Sysmex and flow cytometry results was weak in control, GHT, GDM and MAP groups r: 0570/p<0.01, r: 0.5727/p: 0.0708, r:0.2149/p: 0.4779, r: 0.111/p: 0.779, respectively.
Conclusions: CD34+ cells were significantly higher in the GHT and GDM groups compared with healthy control cord blood. The correlations between Flow cytometry and Sysmex were not strong.

Author Biographies

Enes Mehmet Türkoğlu

Erciyes University School of Medicine, Kayseri, Turkey - Betül-Ziya Eren Genome and Stem Cell Center (GENKOK), Kayseri, Turkey

Şerife Erdem

Betül-Ziya Eren Genome and Stem Cell Center (GENKOK), Kayseri, Turkey - Erciyes University School of Medicine, Medical Biology Department, Kayseri, Turkey

Fatma Zehra Okus

Betül-Ziya Eren Genome and Stem Cell Center (GENKOK), Kayseri, Turkey - Erciyes University School of Medicine, Medical Biology Department, Kayseri, Turkey

Alper Özcan

Erciyes University School of Medicine, Medical Biology Department, Kayseri, Turkey

Zehra Büşra Azizoğlu

Betül-Ziya Eren Genome and Stem Cell Center (GENKOK), Kayseri, Turkey - Erciyes University School of Medicine, Medical Biology Department, Kayseri, Turkey

Ahmet Eken

Betül-Ziya Eren Genome and Stem Cell Center (GENKOK), Kayseri, Turkey - Erciyes University School of Medicine, Medical Biology Department, Kayseri, Turkey

Semih Zeki Uludağ

Department of Obstetrics and Gynecology, School of Medicine, Erciyes University, Kayseri, Turkey

Mehmet Serdar Kutuk

Department of Obstetrics and Gynecology, Medical Faculty, Bezmi Alem University, Istanbul, Turkey

Musa Karakukcu

Erciyes University School of Medicine, Pediatric Hematology-Oncology Department, Kayseri, Turkey

Ekrem Ünal

Betül-Ziya Eren Genome and Stem Cell Center (GENKOK), Kayseri, Turkey - Erciyes University School of Medicine, Pediatric Hematology-Oncology Department, Kayseri, Turkey

Published

2020-09-30

How to Cite

Türkoğlu, E. M., Erdem, Şerife, Okus, F. Z., Özcan, A., Azizoğlu, Z. B., Eken, A., Uludağ, S. Z., Kutuk, M. S., Karakukcu, M., & Ünal, E. (2020). Impact of Intrauterine Growth Restriction Diseases on The Umbilical Cord Blood CD34+ Cell Counts: Morbidly Adherent Placenta and umbilical cord blood CD34+ cells. The Journal of Pediatric Academy, 1(2), 56–61. Retrieved from https://jpediatricacademy.com/index.php/jpa/article/view/66

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